To reconstruct Aβ production in mouse embryonic fibroblasts, wild-variety or mutant Aph1-aL cDNAs ended up inserted into your Eco
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Western blotting showed that the level of Aβ produced by the L30F/T164A mutant was around 2 times the quantity made by the WT (determine 6b). We employed ELISA to quantify Aβ40 plus aβ42 manufacturing through the L30F/T164A mutant; we located that the L30F/T164A mutation resulted in somewhere around two times just as much Aβ40 in addition to aβ42, as opposed Along with the amounts made by the WT (Figure 6c). The ratio of extensive Aβforty two with Aβ40 was comparable concerning the WT and check here mutant (determine 6d).
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